In this study, in order to investigate the effect of seasonal changes on some physiological and biochemical characteristics of POPULUS deltoids, leaf and root samples were collected with four replicates in spring, summer, and autumn in Langroud region in 2010. Results showed that during the summer, with an increase in temperature and decrease in relative humidity, the amounts of chlorophyll a and b, soluble sugars, and activity of catalase and polyphenol oxidase enzymes in leaves were significantly increased compared to the spring leaves, whereas increment in the activity of peroxidase enzyme was not significant. On the other hand, the amount of soluble sugars, activity of catalase and peroxidase enzymes in roots did not show any significant change in the summer compared to the spring roots. However, the activity of polyphenol oxidase enzyme increased significantly. The amount of proline in leaves and roots increased significantly in the spring when the temperature was minimum compared to the other two seasons. The amount of Fe, Cu, and Mn increased significantly both in leaves and roots in spring compared to the other two seasons. Accumulation of Fe and Mn in roots was higher than those of leaves and the accumulation of Cu in leaves was higher than that of the roots in spring and summer.